| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224274 |
| Chromosome: | chromosome 10 |
| Location: | 3494559 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445000 | SLT2 | Sodium/sulfate co-transporter 2; (1 of 2) PF00939//PF02080 - Sodium:sulfate symporter transmembrane region (Na_sulph_symp) // TrkA-C domain (TrkA_C) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGCTCATGATTGTCGCGGGCTTCATC |
| Internal bar code: | GGAACCATACTCGTGAGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 98.4471 |
| LEAP-Seq n confirming: | 10714 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 207 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGGTATGTTCAGCTGGT |
| Suggested primer 2: | TTTGAAGAAAAAGCGGCACT |