| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224538 |
| Chromosome: | chromosome 8 |
| Location: | 4106476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379650 | TIC20 | 20 kDa translocon at the inner membrane of chloroplasts | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGCCGCCGCTGCGAAGCGCTAACCGC |
| Internal bar code: | AAACACGTACTATCAGGCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1194 |
| LEAP-Seq percent confirming: | 99.4384 |
| LEAP-Seq n confirming: | 37361 |
| LEAP-Seq n nonconfirming: | 211 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGATCCTCCCCTGACTC |
| Suggested primer 2: | CGTTCACCATGCACCACTAC |