| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224678 |
| Chromosome: | chromosome 11 |
| Location: | 1653441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467772 | CYCD1 | D-type cyclin; (1 of 10) PTHR10177 - CYCLINE | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACAATGTCATCCCAGCCTCTCGCGCA |
| Internal bar code: | CCCATACCTCGCCGACAAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 87.5256 |
| LEAP-Seq n confirming: | 428 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGTCTGCGACGACGAAT |
| Suggested primer 2: | TCTCAGCAAACACGATGGAG |