| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225539 |
| Chromosome: | chromosome 6 |
| Location: | 6347058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292000 | (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGGCATGGCTGGTCACAAAAGAGAAGA |
| Internal bar code: | TGTGCACGCTGGCTTTCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 90.7495 |
| LEAP-Seq n confirming: | 5533 |
| LEAP-Seq n nonconfirming: | 564 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAAGAGTTCGTAACCCA |
| Suggested primer 2: | AGGACGACCCACTTGTTGAC |