Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.225562 |
Chromosome: | chromosome_4 |
Location: | 675390 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g217951 | LCI33 | Low-CO2-inducible protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGCCCTGCGTGCCTCCTTCTTCTTCTCA |
Internal bar code: | GTGGTTGGCGCGTAGGTGGTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 473 |
LEAP-Seq percent confirming: | 97.0833 |
LEAP-Seq n confirming: | 233 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCACCGTAGGTTGCTGGAC |
Suggested primer 2: | GAGTCAAAGCTAACCGCGTC |