Insertion junction: LMJ.RY0402.225746_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.225746
Chromosome:chromosome_1
Location:4448666
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre01.g030200 PP2C4,PPP3 Phosphoprotein phosphatase 2C delta sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GGCAAACATGTCGTGTGTGTGGCTGTGCGA
Internal bar code:CCATGAAGTGTGTAGACTGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:2175
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTTTGGGAGTTCATCT
Suggested primer 2:TCTGGTTAGCGGTCTTTGCT