| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.225746 |
| Chromosome: | chromosome 9 |
| Location: | 5779090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401997 | (1 of 1) 1.1.1.60 - 2-hydroxy-3-oxopropionate reductase / Tartronate semialdehyde reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTTAGAACCGAGCGAGCGACTCGCGAG |
| Internal bar code: | CCTAACTTGTGTTACCAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.6627 |
| LEAP-Seq n confirming: | 4728 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGAGGAGCAGAGGAGG |
| Suggested primer 2: | CCCTGAAACGTATTGCCACT |