| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226232 |
| Chromosome: | chromosome 12 |
| Location: | 5863891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533750 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCTCCCGATCTCCCACCTGCTCCTCC |
| Internal bar code: | TTCTTTGTCTATCTTGAATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.3488 |
| LEAP-Seq n confirming: | 2441 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCACTTTCTGTCCTCCT |
| Suggested primer 2: | AGCTTCGTAATGATGGGTGG |