| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226637 |
| Chromosome: | chromosome_10 |
| Location: | 4429764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g451600 | sense | CDS|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCTCTGTTGTACACACGCGCCACGCCAGC |
| Internal bar code: | GAATCGGCCAAATAATACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 98.9739 |
| LEAP-Seq n confirming: | 1061 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACTTTCAACCTGCCAAC |
| Suggested primer 2: | TCGCAATCAGAAAAGACACG |