Insertion junction: LMJ.RY0402.226637_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.226637
Chromosome:chromosome 10
Location:4429764
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g451600 SRR28,SRR28B (1 of 1) PF00059//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4); Lectin-domain glycosyl hydrolase CDS|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTCTGTTGTACACACGCGCCACGCCAGC
Internal bar code:GAATCGGCCAAATAATACGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:275
LEAP-Seq percent confirming:98.9739
LEAP-Seq n confirming:1061
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACTTTCAACCTGCCAAC
Suggested primer 2:TCGCAATCAGAAAAGACACG