| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226711 |
| Chromosome: | chromosome 9 |
| Location: | 542357 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403900 | FAP294 | (1 of 5) PF13391 - HNH endonuclease (HNH_2); Coiled-Coil Flagellar Associated Protein 294 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAATGATTGGCGTACAATCGATGCGCG |
| Internal bar code: | CTAGCTACGGAGCTACACATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 99.6569 |
| LEAP-Seq n confirming: | 5228 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCATAGCCTTGCTTAG |
| Suggested primer 2: | GCAACACAACAGCAAGAGGA |