| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226775 |
| Chromosome: | chromosome 12 |
| Location: | 861746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495955 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGGGGCATAAATACGTAGCCTCTAGGG |
| Internal bar code: | CCAACTGCAGCAGGGTCCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGACTGTGTTGTTGGG |
| Suggested primer 2: | CGTAACACCCCTCATCTCGT |