Insertion junction: LMJ.RY0402.226871_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.226871
Chromosome:chromosome 12
Location:1358514
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g488500 ARC6 Accumulation and replication of chloroplast; (1 of 2) PTHR33925//PTHR33925:SF3 - FAMILY NOT NAMED // PROTEIN ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, CHLOROPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACGCCTTGCGAATGGCGTCAGGGCGGCT
Internal bar code:CTCCCAGCTTTTGAATTTGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1025
LEAP-Seq percent confirming:99.669
LEAP-Seq n confirming:16560
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACACATGCTCTCCAAATCCC
Suggested primer 2:AGGACCCCATACTTCCATCC