| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227224 |
| Chromosome: | chromosome 16 |
| Location: | 2582849 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661550 | (1 of 1) PF06941 - 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (NT5C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGCCGCCTCAGCTGTTGTTCGACGCT |
| Internal bar code: | AGATCCCTGTGCGTGGGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 99.3882 |
| LEAP-Seq n confirming: | 12022 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACACGGAAGGAGTGGTCC |
| Suggested primer 2: | TACAATCCACCCCTCTGACC |