| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227417 |
| Chromosome: | chromosome 2 |
| Location: | 7430519 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144550 | (1 of 4) PTHR10110:SF119 - SODIUM/HYDROGEN EXCHANGER 5-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTACCACACACCGTGACACCCAGTGCTA |
| Internal bar code: | ATGGTAGGGCGGCCCTGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 98.4901 |
| LEAP-Seq n confirming: | 4305 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATGCCCTAGTCCTGTGC |
| Suggested primer 2: | TTCCCACCCTATCTTTGTCG |