| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227420 |
| Chromosome: | chromosome 16 |
| Location: | 1268458 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651350 | ATG17 | AuTophaGy related; (1 of 1) PTHR13222//PTHR13222:SF1 - CGTHBA PROTEIN -14 GENE PROTEIN // RB1-INDUCIBLE COILED-COIL PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGACGTTTTAAAAAGGCGCAAAGTCAG |
| Internal bar code: | AGCTAGTCGCCATCAACTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 99.5305 |
| LEAP-Seq n confirming: | 212 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGATACCGTTCTTTGA |
| Suggested primer 2: | TAGCTCACCTTCCGCACTCT |