| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227717 |
| Chromosome: | chromosome 1 |
| Location: | 7503386 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGTATCGTTGCGTACGCGTGCACGTGC |
| Internal bar code: | TCAAGGCAATAGGCCGCTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 98.8949 |
| LEAP-Seq n confirming: | 15481 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 204 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAGAGGAGTAGGGGTGG |
| Suggested primer 2: | GAGGTGTAGCGATGGGTGTT |