| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227739 |
| Chromosome: | chromosome 9 |
| Location: | 4553258 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396139 | (1 of 5) K14326 - regulator of nonsense transcripts 1 (UPF1, RENT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCCGCGCCGCCTCAACGTGGCCATCAC |
| Internal bar code: | CTCCTAGCTAATCCTGGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 29.5933 |
| LEAP-Seq n confirming: | 604 |
| LEAP-Seq n nonconfirming: | 1437 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGAAACGCATAACTTGA |
| Suggested primer 2: | TGGTTGCTTACTGGTGTGGA |