| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227773 |
| Chromosome: | chromosome 3 |
| Location: | 3911829 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171350 | SEC61A | SEC61-alpha subunit of ER-translocon; (1 of 1) K10956 - protein transport protein SEC61 subunit alpha (SEC61A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCTACAGCCTGCTACCCATGCTGCTCC |
| Internal bar code: | TGTCGGCAAGGCAAAAAATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 99.2394 |
| LEAP-Seq n confirming: | 2218 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGAGATTGAGCTGTCCAT |
| Suggested primer 2: | GGTGCGTTTGTTTCCTTGAT |