| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228119 |
| Chromosome: | chromosome 8 |
| Location: | 2047626 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g368450 | (1 of 1) K12667 - oligosaccharyltransferase complex subunit delta (ribophorin II) (SWP1, RPN2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCAACCTGTCCATCCGGCCTCTTCCTC |
| Internal bar code: | AACCGGGGACGTTCGTTATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 999 |
| LEAP-Seq percent confirming: | 99.6582 |
| LEAP-Seq n confirming: | 2041 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGACAGGATGACGACT |
| Suggested primer 2: | TGGAGACGTGAGTGCGATAG |