| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228322 |
| Chromosome: | chromosome 12 |
| Location: | 7539880 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555700 | DNJ15 | DnaJ-like protein; (1 of 5) IPR000104//IPR001623 - Antifreeze protein, type I // DnaJ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACACGATACATGTGGTCACCGGATGTCC |
| Internal bar code: | CCGTCCCGGATGGCCAGTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 97.9667 |
| LEAP-Seq n confirming: | 12479 |
| LEAP-Seq n nonconfirming: | 259 |
| LEAP-Seq n unique pos: | 148 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCTTAAGGGAGAGCTT |
| Suggested primer 2: | ACCAGGGTGTGGTTTACGTC |