Insertion junction: LMJ.RY0402.228332_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.228332
Chromosome:chromosome 5
Location:1209192
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g245351 (1 of 1) PF00059//PF00530//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGTTCTTCTTAGGCTTTCCATCCCTTC
Internal bar code:ACGCCATGCCAAGCACGAGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:597
LEAP-Seq percent confirming:97.3621
LEAP-Seq n confirming:812
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCAGGTTGATGATGAGA
Suggested primer 2:CAGCCTACTGCACCAACTGA