Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.228332 |
Chromosome: | chromosome_5 |
Location: | 1209193 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g245351 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACATACAACGGAGGCACTCAGGGCGCGCCA |
Internal bar code: | ACGAGGTCATCCTTGACATTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 380 |
LEAP-Seq percent confirming: | 99.6136 |
LEAP-Seq n confirming: | 4383 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGCAGGTTGATGATGAGA |
Suggested primer 2: | CAGCCTACTGCACCAACTGA |