| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228378 |
| Chromosome: | chromosome_12 |
| Location: | 9159132 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g542400 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTTGAAGTGACTGGCGTTGCCTGGTGTTC |
| Internal bar code: | CGACGCTCGCGAGTTCCAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.375 |
| LEAP-Seq n confirming: | 318 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCAGGGAGACCTAGTGC |
| Suggested primer 2: | ACAAAGCCGGGTCACACTAC |