Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.228556 |
Chromosome: | chromosome_3 |
Location: | 3050044 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g164250 | FAP262 | Flagellar Associated Protein, protein kinase | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGGTGGAAGTCGCTGCTGCGGAGTAGGCG |
Internal bar code: | CTGCCCGCCAAGAAATACGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 709 |
LEAP-Seq percent confirming: | 98.9317 |
LEAP-Seq n confirming: | 15095 |
LEAP-Seq n nonconfirming: | 163 |
LEAP-Seq n unique pos: | 200 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACCTGGAGCTGGACTTCCT |
Suggested primer 2: | GAGACTGCCCAGAAGCAATC |