| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228800 |
| Chromosome: | chromosome 1 |
| Location: | 5429032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038151 | (1 of 1) PTHR23069:SF0 - TAT-BINDING HOMOLOG 7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCCTTACGGTACACCCTACCGGTGCG |
| Internal bar code: | GACAACCCCCGTGTTCAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 78.9474 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTTATTTCGTTGTTCGC |
| Suggested primer 2: | TGCTGCTGTGGATTCTTACG |