| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228887 |
| Chromosome: | chromosome 4 |
| Location: | 1777044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212100 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTATACCCGGCTCAAAAAGGCTGTGGTCA |
| Internal bar code: | ATTTTAACAGCGGTATTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 99.1543 |
| LEAP-Seq n confirming: | 469 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAACCAAATCAGCGAAG |
| Suggested primer 2: | GAGGCAACCACTCAGCTAGG |