| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229313 |
| Chromosome: | chromosome 1 |
| Location: | 5049486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034900 | CDPK5 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1; Calcium/calmodulin-dependent protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGAAATTGATTGCCCACATCCTCACGTT |
| Internal bar code: | TTGCCTGGAGTCCATGCACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 96.9432 |
| LEAP-Seq n confirming: | 222 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGTGATATCTGCCCCT |
| Suggested primer 2: | TCTGAACTTTTGCGGGTCTT |