| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229401 |
| Chromosome: | chromosome 10 |
| Location: | 1400045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCCACAACCGCCATCGGTCGGCTCCCT |
| Internal bar code: | CAATTTAAGGGTGCCGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 98.8682 |
| LEAP-Seq n confirming: | 27778 |
| LEAP-Seq n nonconfirming: | 318 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGTGTGCTTTGGAATGG |
| Suggested primer 2: | GTCAACGTGCGGTAGGAAAT |