| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229617 |
| Chromosome: | chromosome_1 |
| Location: | 2406543 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g013300 | DEG10 | DegP-type protease | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATAATCATGAATGTGACCCCCTTCCCTTAT |
| Internal bar code: | GCGTATTAGCTGACACGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 95.092 |
| LEAP-Seq n confirming: | 310 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTGTCAGAACCTGGAG |
| Suggested primer 2: | AGCCTCAGTGCCTCTCACAT |