Insertion junction: LMJ.RY0402.229619_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.229619
Chromosome:chromosome 1
Location:483463
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTAAGAAAGGCGACCGAGCGCTTGTGC
Internal bar code:CGGATGAATCCCAGCAGGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:99.3311
LEAP-Seq n confirming:2376
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGCAGATGGTGGACACG
Suggested primer 2:TAAGGGACAGAGCCAACTCG