| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229620 |
| Chromosome: | chromosome 7 |
| Location: | 2280446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327700 | (1 of 2) PTHR24161//PTHR24161:SF17 - FAMILY NOT NAMED // PALMITOYLTRANSFERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGCCCTTCGCCCGGGCTGTGCTGTTG |
| Internal bar code: | GCACCGCTGGAGACGCCAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1128 |
| LEAP-Seq percent confirming: | 99.4129 |
| LEAP-Seq n confirming: | 5757 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGGTAACTGGAATGTCG |
| Suggested primer 2: | TCCAACTGCTTCACGTTCTG |