| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229701 |
| Chromosome: | chromosome 16 |
| Location: | 3201032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666334 | RFS1 | (1 of 1) 2.4.1.82 - Galactinol--sucrose galactosyltransferase / Raffinose synthase; putative raffinose synthase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCCACACGGAGCTGCCCTTCCCGCCT |
| Internal bar code: | GGAGCGAATGTCAAAGGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 99.2126 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACATCCCCTCAAACCCCT |
| Suggested primer 2: | GATGCCGGAGCTCTGTAGAC |