| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229927 |
| Chromosome: | chromosome 6 |
| Location: | 4973970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279850 | Tic62-NAD-6 | (1 of 1) PTHR14194//PTHR14194:SF78 - NITROGEN METABOLIC REGULATION PROTEIN NMR-RELATED // SUBFAMILY NOT NAMED; Putative dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGTGTCGGGGAGCCCCGCCAGCTTGG |
| Internal bar code: | TTATGCAAACAGTCGAAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 91.9753 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACCTCCTCTGCTCCAAC |
| Suggested primer 2: | TTGGGCTTTTACCTACGTGC |