Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.229927 |
Chromosome: | chromosome 9 |
Location: | 3276680 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g388060 | VDR1 | violaxanthin de-epoxidase related, chloroplast; (1 of 1) 1.10.99.3 - Transferred entry: 1.23.5.1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCTGGCGTTGTTGTGTGCCACCCGGC |
Internal bar code: | TCCCGTTGGCGCTAGTTTCTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 401 |
LEAP-Seq percent confirming: | 99.7611 |
LEAP-Seq n confirming: | 1253 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATATGGCAGGAAGCGTTGAC |
Suggested primer 2: | GCAGGAAATCGATGCTTCTC |