Insertion junction: LMJ.RY0402.229927_5

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.229927
Chromosome:chromosome 9
Location:3276680
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g388060 VDR1 violaxanthin de-epoxidase related, chloroplast; (1 of 1) 1.10.99.3 - Transferred entry: 1.23.5.1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGCTGGCGTTGTTGTGTGCCACCCGGC
Internal bar code:TCCCGTTGGCGCTAGTTTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:99.7611
LEAP-Seq n confirming:1253
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATATGGCAGGAAGCGTTGAC
Suggested primer 2:GCAGGAAATCGATGCTTCTC