| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229992 |
| Chromosome: | chromosome 4 |
| Location: | 2659322 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223050 | CAH2 | (1 of 2) PTHR18952:SF104 - CARBONIC ANHYDRASE-RELATED PROTEIN; Carbonic anhydrase, alpha type%252C periplasmic | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGACTGAAGCATTTTGCCTGCCTGCCTC |
| Internal bar code: | CTTGATAGAGCAGGCATCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 99.4325 |
| LEAP-Seq n confirming: | 8761 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGAAGTGGAACTGGGTG |
| Suggested primer 2: | CAGGTGAGTAGCCAGCATGA |