| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230157 |
| Chromosome: | chromosome 16 |
| Location: | 2593948 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661626 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGATGGCTGACTGGCCAGGCTCTCGAAC |
| Internal bar code: | GGTTTCCGTTTGAGCCGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 67.963 |
| LEAP-Seq n confirming: | 367 |
| LEAP-Seq n nonconfirming: | 173 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCCTCGGTTTGATAGC |
| Suggested primer 2: | AGAACTGGATTCATGTCCGC |