| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230371 |
| Chromosome: | chromosome 6 |
| Location: | 3855318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278174 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTACCTGCCCCATGCAGCAGCCCCATGC |
| Internal bar code: | GCGTACCACCAGTCGTTACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 99.6633 |
| LEAP-Seq n confirming: | 592 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCACCAGCTGTGCTAGA |
| Suggested primer 2: | CATGCACAGTATACGCCCAC |