Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.230873 |
Chromosome: | chromosome_12 |
Location: | 8962230 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g544114 | CYN20-6 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAGGCATTCAGAGTTTGGGGTTGAGCAGGA |
Internal bar code: | GAGGCTAACGCTAAACGGGTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 422 |
LEAP-Seq percent confirming: | 92.8571 |
LEAP-Seq n confirming: | 39 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGTAACCGCCAGTACCAT |
Suggested primer 2: | CGAGAAGAAGAATGGCCTTG |