Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.231268 |
Chromosome: | chromosome_13 |
Location: | 2134076 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g577950 | VPS60 | Subunit of the ESCRT-III complex | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGGGAATAGCTGAGAGCAGATCCCAACA |
Internal bar code: | TCTGGATATGAAGGACAATTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 465 |
LEAP-Seq percent confirming: | 99.1054 |
LEAP-Seq n confirming: | 2991 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTGTTCTTCTTGAAGGCGG |
Suggested primer 2: | CATGTATCAGTCGGACACGG |