Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.231469 |
Chromosome: | chromosome_3 |
Location: | 3047862 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g164250 | FAP262 | Flagellar Associated Protein, protein kinase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGGTATCTACAGCCCCATCCCCCGCCTGC |
Internal bar code: | TTATAGCGTACGGTAAATTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 660 |
LEAP-Seq percent confirming: | 80.8025 |
LEAP-Seq n confirming: | 5417 |
LEAP-Seq n nonconfirming: | 1287 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATGGTGATGAAGTGCAAGG |
Suggested primer 2: | GTCGCGGTACACCATTTTCT |