Insertion junction: LMJ.RY0402.231469_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.231469
Chromosome:chromosome_3
Location:3047865
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre03.g164250 FAP262 Flagellar Associated Protein, protein kinase sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AAACGACAGGGGATGGGGCGGGGGATGGGG
Internal bar code:GTGCTGCCTCGGGCGTATGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:215
LEAP-Seq percent confirming:89.0909
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CATGGTGATGAAGTGCAAGG
Suggested primer 2:GTCGCGGTACACCATTTTCT