| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231484 |
| Chromosome: | chromosome 4 |
| Location: | 3151449 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226050 | ARS10 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACCCCCAAACCGGCCGGCGCAGGAAAG |
| Internal bar code: | AGCCGACATCGTTGTCGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 99.0629 |
| LEAP-Seq n confirming: | 1480 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCGTTCATTGGTGTAG |
| Suggested primer 2: | ACGACATAAAACACCGCACA |