| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231640 |
| Chromosome: | chromosome 14 |
| Location: | 1771260 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619825 | (1 of 2) PTHR10848 - MEIOTIC RECOMBINATION PROTEIN SPO11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCCCGCACCCGACTCCACCTCCGTCTC |
| Internal bar code: | GGATATCATTCCATATTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.0256 |
| LEAP-Seq n confirming: | 813 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCCTCCTGGTTCAGTGG |
| Suggested primer 2: | CAACAAAACGAATGTGTCCG |