| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231713 |
| Chromosome: | chromosome 12 |
| Location: | 9068203 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542950 | CGL137,NUP54 | Nucleoporin 54; (1 of 1) K14308 - nuclear pore complex protein Nup54 (NUP54, NUP57) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACTCACTTTGACCCCAAACATCTTGGT |
| Internal bar code: | TTCTGAACGCTATTAAATGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 99.2542 |
| LEAP-Seq n confirming: | 3194 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGCTGTGGCACTCATTT |
| Suggested primer 2: | AAGCAGTATTGAACGGGGTG |