| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232738 |
| Chromosome: | chromosome 12 |
| Location: | 5112811 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526883 | (1 of 18) PF12906 - RING-variant domain (RINGv) | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCAGCTGCCCCGCCGGGCGCTCCAGAC |
| Internal bar code: | TTTCGGAGGCCAGTAAAGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 73.913 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGCTCTGAACAAGTGGG |
| Suggested primer 2: | CCAAAACACTGATGCCAATG |