Insertion junction: LMJ.RY0402.233077_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.233077
Chromosome:chromosome 1
Location:7338672
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g052750 (1 of 1) PF06011//PF13418 - Transient receptor potential (TRP) ion channel (TRP) // Galactose oxidase, central domain (Kelch_4); Transient receptor potential ion channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGTCGTACTTCTGGGTGCGTGCCGTA
Internal bar code:ATATCGTTTATTGCTAACTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:866
LEAP-Seq percent confirming:99.4306
LEAP-Seq n confirming:13795
LEAP-Seq n nonconfirming:79
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTGTGTGTGTGTGTGT
Suggested primer 2:CCAGGTGAGTTGTCGGTTTT