Insertion junction: LMJ.RY0402.233454_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.233454
Chromosome:chromosome 6
Location:8242165
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g305900 CPLD22,UAA4 Putative GDP-fucose transporter, conserved in the plant lineage and diatoms; (1 of 3) PTHR11132//PTHR11132:SF64 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTAATGCGCCCCTGGCACTAGCAACTAC
Internal bar code:TACCATACTCGCTGCGCTAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:74.8243
LEAP-Seq n confirming:8304
LEAP-Seq n nonconfirming:2794
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGCTTATGGGACAGGGA
Suggested primer 2:CTGGAATTGTATTTCGGCGT