Insertion junction: LMJ.RY0402.233992_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233992
Chromosome:chromosome 13
Location:2669631
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g581850 (1 of 1) PTHR10566//PTHR10566:SF53 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTATTTGGATGTGCGACTTGGCTTGCGCG
Internal bar code:TACCCACGAAGTGAACGCCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:595
LEAP-Seq percent confirming:98.6326
LEAP-Seq n confirming:1659
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGCCTGCCCTAGCAACAC
Suggested primer 2:CACTCTAATGCGCAACTCCA