| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234610 |
| Chromosome: | chromosome 12 |
| Location: | 4572583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522400 | (1 of 1) PTHR10641:SF538 - LD18233P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATGTCGCTGCTGGCCCGGCTTCAAGCT |
| Internal bar code: | CCACGGGGGAAGGCACGACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 89.3787 |
| LEAP-Seq n confirming: | 1683 |
| LEAP-Seq n nonconfirming: | 200 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTACGCTGTTGATGCGTT |
| Suggested primer 2: | CGGATGTCCTTGTGTGTGTC |