| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234711 |
| Chromosome: | chromosome 7 |
| Location: | 6272852 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356750 | (1 of 2) PTHR10926 - CELL CYCLE CONTROL PROTEIN 50 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCACACCGTCGCCCGGCCATTGTTTTGC |
| Internal bar code: | TATACTTGGTGAGACTCTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 403 |
| LEAP-Seq percent confirming: | 57.3036 |
| LEAP-Seq n confirming: | 5551 |
| LEAP-Seq n nonconfirming: | 4136 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACACACACACACACAC |
| Suggested primer 2: | CTGTGTAGGAGGCTTGGAGC |